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12. Hu, Y. S.; Wang, Z. G.; Fu, H. H.; Zhou, C. Z.; Cai, W. S.; Shao, X. G.; Liu, S. L.; Pang, D. W.*, In-Situ Synthesis of Quantum Dots in the Nucleus of Live Cells. Natl. Sci. Rev. 2024, 11(3). DOI: 10.1093/nsr/nwae021.

Enhanced fluorescence in the nucleus after the addition of GSH. (a) The procedure for generating fluorescent cells by treatment with sodium selenite (Na2SeO3), cadmium chloride (CdCl2) and glutathione (GSH). (b) Time-lapse fluorescence images of Madin-Darby canine kidney (MDCK) cells treated with Se’ and Cd’ and different concentrations of GSH. (c) Fluorescence images of the treated cells stained with nucleus dye (the first column), and Hoechst 33342 (the second column). (d) The fluorescence and differential interference contrast (DIC) images of a nucleus extracted from treated cells. (e, f) The fluorescence intensity of the nucleus (e) and cytoplasm (f) of cells treated with Se’ and Cd’ and different concentrations of GSH. (g) A heat map of the fluorescence intensity of the nucleus and cytoplasm in (e) and (f). (h) Fluorescence images of Se’- and Cd’-treated, and Se’-, Cd’- and GSH-treated MDCK cells 6 h after the addition of CdCl2. The insets show the line profiles of the fluorescence intensity along the dashed lines showed in the images. All imaging data (b, e–g) represent means ± SD of triplicate measurements in multiple cells. n = 20 for (e) and (g) cells. Se’ stands for Na2SeO3; Cd’ stands for CdCl2.


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